![]() ![]() "Loamy," which many Wordle users tried back in August, though the right word that day was "clown. "LGBTQIA," for lesbian, gay, bisexual, transgender, queer or questioning, intersex, and asexual, aromantic or agender. "Sentient," with lookups brought on by Google canning the engineer who claimed an unreleased AI system had become sentient. "Raid," as in the search of former President Donald Trump's Mar-a-Lago home. "Queen consort," what King Charles' wife, Camilla is newly known as. "Codify," as in turning abortion rights into federal law. ![]() "Omicron," the persistent COVID-19 variant and the 15th letter of the Greek alphabet. "Oligarch," driven by Russia's invasion of Ukraine. Last year's pick was "vaccine." Rounding out this year's Top 10 are: "Gaslighting," Sokolowski said, spent all of 2022 in the top 50 words looked up on to earn top dog word of the year status. Some of the droves who looked up "gaslighting" this year might have wanted to know, simply, if it's one or two words, or whether it's hyphenated. They don't slice and dice why people look up words, which can be anything from quick spelling and definition checks to some sort of attempt at inspiration or motivation. Sokolowski and his team weed out evergreen words most commonly looked up to gauge which word received a significant bump over the year before. Merriam-Webster, which logs 100 million page views a month on its site, chooses its word of the year based solely on data. Among other instances, he insists her complains over the constant dimming of their London townhouse's gaslights is a figment of her troubled mind. The two marry after a whirlwind romance and Gregory turns out to be a champion gaslighter. Full Review George Cukor entered Hitchcock territory with this shadowy tale of suspense and madness set in the gas-lit glow and cobblestone quaintness of Victorian London. One, George Cukor's "Gaslight" in 1944, starred Ingrid Bergman as Paula Alquist and Charles Boyer as Gregory Anton. It birthed two film adaptations in the 1940s. There's also "medical gaslighting," when a health care professional dismisses a patient's symptoms or illness as "all in your head."ĭespite its relatively recent prominence - including "Gaslighter," The Chicks' 2020 album featuring the rousingly angry titular single - the word was brought to life more than 80 years ago with "Gas Light," a 1938 play by Patrick Hamilton. After her fathers passing, she receives a. It can be a corporate tactic, or a way to mislead the public. Shes a successful Washington DC architect and happily married. It can happen between romantic partners, within a broader family unit and among friends. ![]() Gaslighting is a heinous tool frequently used by abusers in relationships - and by politicians and other newsmakers. ![]()
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![]() Necessary cookies are absolutely essential for the website to function properly. The right combination of antibodies targeted to strategic epitopes will bring clarity to the state of any intracellular signaling cascade. The antibody selection guide will lead you to the best options for single-purpose antibodies or those that you will use in multiple applications. ![]() It takes into consideration the specimen species, the host species, the desired level of specificity, and cross-reactivity concerns, among other factors. Our antibody selection guide makes picking the right combination of antibodies easy. Single domain antibodies are also useful for these experiments, which add to the versatility of combinations of antibodies that can be used in the same assay.īoth monoclonal and polyclonal antibodies are useful in detecting the state of intracellular signaling cascades. It is thus important that the right host species for primary and secondary antibodies are considered with forethought. Multiple epitopes, whether on the same protein or different proteins, can be simultaneously or sequentially detected on the same blot or same cells. Since a protein can have multiple phosphorylation sites, each of which affects the protein differently, in addition to multiple sites for moieties such as ubiquitination, picking the right epitopes to detect is a fruitful – but nontrivial – task. Phosphorylated epitopes are often targets of immunoblotting and in situ immunolocalization assays. Thus, antibodies that are designed to detect the presence or absence of a chemical moiety on a signaling protein can give researchers a clear picture of what pathway has been activated and to what extent. Each active conformation of a protein or phosphorylation site can serve as a different epitope that can be bound by an antibody. The aforementioned complexity of intracellular signaling is precisely why picking the right antibodies is crucial when researchers seek to accurately determine the state of a cell in response to extracellular stimuli. Picking the Right Epitopes for Accurate Information The ability of scaffold proteins to bind MEK and ERK is also regulated in terms of the scaffold’s activation state, which changes after post-translational modification by the addition of chemical moieties. Proteins such as MEK and ERK are known to bind near each other on scaffold proteins. For example, phosphorylated MEK or ERK is often interpreted as representing activation of an intracellular signaling pathway downstream of a GPCR.įurthermore, these signaling proteins can have multiple sites of phosphorylation, different combinations of which affect the level of the protein’s activity or half-life. The activation state of a signaling protein is often used as an indicator of whether a certain pathway has been activated. They also depend on the presence or absence of moieties, such as phosphate groups, ubiquitin groups, and calcium ions. These states depend on conformational changes that result from interactions with other proteins. The complexity of intracellular signaling is that the proteins in a signaling cascade have varying states of activity (i.e. Activation States of the Signaling Cascade ![]() ![]() ![]() Josephine Barlow Bistline, LaDell Bistline Jr., Brenda Barlow, Marona Johnson, Torrance Bistline, Leia Bistline, and Leilani Bistline were added to the indictment on May 18. Attorney's Office filed affidavits describing sexual coercion but had not filed charges against them. Originally, the four faced charges of kidnapping and destruction of evidence in a federal investigation. Indictment: Read the 56-page redacted second superseding indictmentīateman had been charged in December, along with Naomi Bistline, Donnae Barlow and Moretta Johnson, all of whom Bateman claimed to be his wives. The new indictment revealed stories of Bateman coercing little girls to have sex with him and other adults, trading nights with girls for luxury Bentley cars, coercing children into participating in live-streamed group sex acts and transporting across state lines the girls for sexual trade. The new accusations rely, in part, on Bateman's own writings and remarks from some of the girls, according to documents filed in U.S. The latest court filings, naming 11 defendants, show the scope of the case has widened and the allegations have grown more serious. Attorney's Office for Arizona now accuses Bateman, the self-proclaimed prophet of a splinter group of the Fundamentalist Church of Jesus Christ of Latter Day Saints, and 10 others of causing sexual harm to nine girls whose ages ranged from 9 to 17 at the time the alleged offenses took place.įederal prosecutors filed the extensive indictment on May 18, replacing a more limited one against Bateman and three followers. PHOENIX - Lurid new details have emerged from a new 53-felony-count federal indictment against an Arizona-based polygamist fundamentalist cult and its leader, Samuel Bateman. Watch Video: Today in History for May 26th ![]() ![]() ![]() During the fourth quarter, we continued to drive productivity and efficiency improvements across the organization, consistent with the plans shared with you last quarter. The new branch is now complete and we will be open in March. ![]() Last summer, we opened a temporary location while our new facilities were constructed. As previously disclosed, we are expanding into Lubbock, Texas. Northern Texas has a strong backlog of infrastructure, commercial and industrial projects, which are bolstering demand for Rabern Rentals. Our rental segment, which is represented by Rabern, performed ahead of expectations during the fourth quarter, supported by robust rental revenue within the Texas market. We continue to see strong demand in our lifting equipment in both North America and Europe driven by elevated activity levels across key end markets such as transportation and infrastructure, upstream energy and electrical distribution as well as general construction. Excluding the revenue contribution from Rabern Rentals, the acquisition we completed in April of 2022, we generated organic revenue growth of 34% in the fourth quarter driven by the strength of our core lifting segment. Fourth quarter revenue increased 48% on a year-over-year basis. Our team delivered strong fourth quarter results, highlighted by significant organic revenue growth across our lifting equipment and rental segments, sustained margin expansion and our fourth consecutive quarter of improved profitability. Michael Coffey: Thank you, Paul and good morning to everyone joining us on the call today. With that, I will turn the call over to Mike. Today's call will begin with prepared remarks from CEO, Michael Coffee, who will provide a review of our recent business performance, including an introduction of our new elevating excellence initiative, followed by a financial update and outlook for our CFO, Joseph Doolan.Īt the conclusion of these prepared remarks, we will open the line for questions. Additionally, please note that you can find reconciliations of historical non-GAAP financial measures in the press release issued earlier today and in the appendix of this presentation. For a discussion of some of the factors that could cause actual results to differ please refer to the Risk Factors section of our latest filings with the SEC. This release together with the accompanying presentation materials, are publicly available in the Investor Relations section of our corporate website at I would like to remind you that management's commentary and responses to questions on today's conference call may include forward-looking statements, which by their nature are uncertain and outside of the company's control.Īlthough these forward-looking statements are based on management's current expectations and beliefs, actual results could differ materially. We issued a press release earlier today detailing our fourth quarter and full year operational and financial results. Leading the call today are CEO, Michael Coffey and Chief Financial Officer, Joseph Doolan. Welcome to Manitex International's fourth quarter and full year 2022 results conference call. ![]() I will now turn the conference over to Paul Bartolai, Managing Director with Vallum Advisors. Please note this conference is being recorded. Fourth Quarter and Full Year 2022 Results Conference Call. (NASDAQ: MNTX) Q4 2022 Earnings Call Transcript March 8, 2023 ![]() |